目的:旨在调查卵圆孔未闭（PFO）封堵术后血清标志物的变化并探讨其临床意义。方法:本研究将回顾性纳入2019年12月到2022年10月期间在北京阜外医院行PFO封堵术的患者210例。分别测量患者封堵前后的血清标志物水平，包括：中性粒细胞与淋巴细胞的比值、血小板绝对值与淋巴细胞绝对计数的比值、平均血小板体积、血浆同型半胱氨酸、总胆固醇以及高密度脂蛋白胆固醇水平。比较患者治疗前后血清标志物的变化情况。将卵圆孔未闭并发脑卒中的患者分为病例组，本院同时期入住的149例房间隔缺损患者作为对照组。病例组在封堵术前后分别与对照组进行血液指标水平的比较。结果:封堵术后患者中性粒细胞与淋巴细胞的比值、血小板绝对值与淋巴细胞绝对计数的比值、平均血小板体积均高于封堵术前，血浆Hcy、总胆固醇、高密度脂蛋白胆固醇水平均低于封堵术前，差异均有统计学意义（均P＜0.05）。PFO封堵术前病例组的血浆Hcy水平明显高于对照组，差异有统计学意义（P＜0.01），封堵术后病例组的血浆Hcy水平与对照组比较，差异无统计学意义。结论：卵圆孔未闭封堵术后同型半胱氨酸水平显著降低，高同型半胱氨酸水平可能与PFO并发卒中密切相关。综合来看，对于卵圆孔未闭的患者，监测其同型半胱氨酸水平是有临床意义的。背景：在介入手术中，心脏血管造影被认为是一种安全且有效的方法，用于诊断和评估先天性心脏病。在这项研究中，我们探讨了卵圆窝血管造影（FOA）在诊断卵圆孔未闭（PFO）（伴隐源性卒中和/或偏头痛）和评估房间隔解剖结构方面的疗效。方法：本研究对88例在我院接受卵圆窝血管造影（FOA）和经皮PFO封堵术的患者进行了回顾性分析。探讨了卵圆窝造影在卵圆孔未闭检测和评估房间隔解剖中的价值。结果：卵圆窝造影的敏感性、特异性、阳性预测值和阴性预测值分别为97.7%、100%、100%和50%。根据血管造影的形态计量学评估，PFO可分为袋状PFO（n=12），即具有房间隔袋的PFO和隧道状PFO (n=72)。与隧道状PFO相比，袋状PFO具有更高的多开口PFO发生率（P=0.019）、憩室存在率（P=0.009）和造影剂滞留率（P<0.001）。在手术过程中，在袋状PFO中观察到更高的荧光透视时间（P=0.036）。血管造影证实导丝可以滑入间隔血管。血管造影术期间和术后均未观察到并发症。结论：FOA在评估PFO方面具有重要作用，尤其是在易于识别间隔囊袋、揭示其精细细节以及识别间隔血管方面。因此，卵圆窝血管造影可作为闭合前确认和评估PFO的替代方法。目的：本研究旨在评估经导管卵圆孔未闭缝合器系统(HaloStitch®)在动物（猪）模型中的可行性、安全性和有效性。方法：穿刺13只实验动物造出卵圆孔未闭模型，并植入新型经导管卵圆孔未闭缝合器系统，观察手术成功率。并在6个月的随访期间获得超声、病理、大体标本数据。结果:成功植入缝合器11只。心脏大体解剖显示，所有缝合器均稳定在房间隔内，表面未见赘生物或血栓形成。超声结果显示在术后长期随访过程中，锁钉的位置未发生改变，缝合线未发生断裂，未见赘生物和血栓，锁钉在超声下显影清晰。房间隔缺损口直径和房间隔残余分流流速随观察时间增加而明显减少。病理观察表明，植入物头部可见部分机化的血栓，高分子材料可见纤维结缔组织包裹，有炎性细胞浸润。结论：本研究结果证明了经导管卵圆孔未闭缝合器系统(HaloStitch®)操作可行性良好，在猪动物模型的安全性良好和材料生物相容性良好。

Objective: The study aimed to investigate changes in serum biomarkers after Patent Foramen Ovale (PFO) closure and explore their clinical significance.Methods: This study retrospectively enrolled 210 patients who underwent Patent Foramen Ovale (PFO) closure procedures at our institution from December 2019 to October 2022. Serum biomarkers before and after the closure were measured, including the neutrophil/ lymphocyte (NLR), the platelet/ lymphocyte (PLR), mean platelet volume (MPV), plasma homocysteine (Hcy),total cholesterol, and high-density lipoprotein cholesterol levels. Changes in serum biomarkers before and after treatment were compared. Patients with PFO complicated by cryptogenic stroke were classified into a subgroup (case group), while 149 patients with atrial septal defect admitted during the same period served as the control group. The levels of blood indicators before and after the closure surgery were compared between the case group and the control group.Results: The levels of NLR, PLR, and MPV in patients after occlusion were higher than before, while the levels of plasma Hcy, total cholesterol, and high-density lipoprotein cholesterol were lower than before occlusion, and the differences were statistically significant (all P<0.05). There was a significant difference in the levels of homocysteine between the case group and the control group before the PFO closure (P＜0.01), with the case group having significantly higher Hcy levels than the control group. After PFO closure, the difference in homocysteine levels between the case group and the control group was not statistically significant.Conclusion: The levels of homocysteine significantly decreased after PFO closure, and high levels of Hcy may be closely related to PFO-associated cryptogenic stroke. Overall, monitoring homocysteine levels in patients with PFO is beneficial.Background: Cardiac angiography during interventional procedures was a safe and efficient way to diagnose and evaluate congenital heart disease. In the study, we explored the efficacy of Foramen Ovalis Angiography (FOA) in diagnosing Patent Foramen Ovale (PFO) (with cryptogenic stroke and/or migraine) and assessing atrial septal anatomy.Methods: This study conducted a retrospective analysis of 88 patients who underwent Foramen Ovale Angiography (FOA) and percutaneous closure of PFO in our hospital. Values of FOA in detecting PFO and assessing septal anatomy were analyzed.Results: Sensitivity, specialty, positive predictive value, and negative predictive value of FOA were 97.7%, 100%, 100%, and 50%. Under the morphometric assessment of angiography, PFO could be divided into Pouch-like PFO(n=12), which referred as PFO with atrial septal pouch, tunnel-like PFO (n=72). Different from tunnel-like PFO, Pouch-like PFO has a higher rate of multi-opening PFO (P=0.019), presence of diverticulum (P=0.019), contrast medium retention (P<0.001). higher fluoroscopic time was observed in pouch-like PFO during the procedure (P=0.036). Angiography confirmed guidewire can slip into the septal vessel. No complications were observed during and after angiography.Conclusion: FOA has a major role in assessing PFO, especially for easier identification of septal pouch, presenting its fine details, and recognizing septal vessels. Thus, Foramen Ovalis Angiography could be used as an alternative method to confirm and evaluate PFO before closure.Objectives: This study aimed to evaluate the feasibility, safety, and efficacy of a novel transcatheter suture closure system (HaloStitch®) for Patent Foramen Ovale (PFO) closure in a swine model.Methods: Thirteen swine were selected for the experimental creation of a PFO model. They underwent implantation of the transcatheter suture closure system to evaluate the procedure's success rate. Comprehensive follow-up over six months included ultrasound imaging, histopathological analysis, and examination of gross cardiac specimens.Results: Eleven of the thirteen swine successfully received the HaloStitch® device implant. Examination of Gross cardiac anatomy revealed that all sutures were securely positioned in the atrial septum without evidence of redundancy or thrombosis. Ultrasound findings indicated stable suture and staple positions over the extended postoperative period, with no signs of suture breakage, redundancy, or thrombus formation. Additionally, ultrasound demonstrated clear visualization of the staples. The diameter of the atrial septal defect orifice and the velocity of residual septal shunt flow markedly decreased over the observation period. Histopathological observations showed that the presence of partially organized thrombi at the implant’s head and fibrous connective tissue encapsulation with inflammatory cell infiltration around the polymer material.Conclusions: The study demonstrate that the transcatheter suture closure system (HaloStitch®) for PFO closure is feasible, safe, and biocompatible in a swine model, showing promising potential for human application.